Differential Effects of Bisphenol A and Di (2-Ethylhexyl) Phthalate Exposure on Crestin and the Expression of Some Genes Related to Apoptosis and Inflammation in Zebrafish Embryos

Objective: Endocrine disrupting chemicals (EDC) in plastics may disrupt proper endocrine system functioning. Zebrafish embryos are formed through external fertilization, and their rapid development, short life cycle, transparency provide imaging advantages. In zebrafish embryos, neural crest development occurs similarly to other vertebrate crestin is found the during embryogenesis. The aim of our study evaluate effects bisphenol A (BPA) Di (2-ethylhexyl) phthalate (DEHP), which most widely used EDCs, on expression crestin, apoptosis, inflammation-related parameters embryos. Materials Methods: were exposed either DEHP or BPA well plates for 72 h post fertilization (hpf). Expressions evaluated by situ hybridization, while expressions bax, casp8, casp3a, ifng1, fas, tp53 RT-PCR. Results: bax casp8 increased fas decreased groups. group but compared with control group. group, No significant change was observed When an inverse relation between ifng1 as evidenced expressions, Conclusion: Our provided important data EDCs relationship inflammation apoptosis.